2. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Louis, MO, USA). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. All configurations. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Dissolve FITC-dextran in PBS at a. 1. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4To quantify F-CHP fluorescence, stained samples were digested in 500 μl of proteinase K (1 mg/ml) in water for 3 hours at 60°C. Multi-Mode Microplate Readers. FP assay do not perform well with protein:protein interactions. 161 likes. Plate Formats: 6, 12, 24, 48, 96, 384 Well Plates. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. All configurations. 4. The percentage of viability of each treatment group in different. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). Note: In this user guide, SpectraMax ® refers to several SpectraMax . Then cells were collected, and the caspase-3 expression level was detected with the caspase-3 assay kit. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). The IC50 curves were calculated using variable slope for log (inhibitor) vs. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). SigmaPlot (Systat Software, Inc. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). Microplate Reader Shelf. 2 pM 2 pM 384-well (50 µL) 3 pM 4 pMCell viability was determined by addition of MTT ([3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma) to cells and incubation for 4 h. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 5. The fluorescent signal at an excitation wavelength of 530 nm and emission wavelength of 580 nm was measured by Spectramax M4, USA. qPCR The jejunum was sampled immediately after termination, snap frozen, andChoose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 2. No. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. normalized response (Graphpad Prism 8. ITEM#: M4-MOL Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. Fluorescence Microplate Reader Comparison Chart. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. 1. 37. ® reader to the computer. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. We show that a ML model, trained on the. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax® M4 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. Establishment of mouse orthotopic xenograft bladder tumor model. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. Example: [Drawer]. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. FlexStation ®, SpectraMax. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). Choose from a three- (M3), four- (M4), orLuciferase Detection Instrumentation Guide. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. My List.